What does the Spin Method rely on for separation of sperm?

The Spin Method utilizes the principle of density based on the genetic content of sperm to achieve separation. The correct reasoning behind why heavy X chromosomes and lighter Y chromosomes behave differently during the spinning process is key to understanding this method. In this technique, sperm cells are subjected to a centrifugation process where heavier X chromosome-bearing sperm move outward to the edge of the centrifuge due to their increased mass, while the lighter Y chromosome-bearing sperm tend to remain closer to the center.

This separation mechanism is vital for applications such as gender selection in assisted reproductive technologies, as it allows for the selection of sperm that carry a specific chromosome. The effectiveness of the Spin Method in providing a means to preferentially select X or Y chromosome-bearing sperm hinges on the principles of density and centrifugal force rather than the other factors listed.